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      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control

      eDNA Isolation Kit - With or Without Internal Positive Control

      Everything about Isolation
      Brand: Sylphium
      €100,43
      • Including 21% Tax: €100,43
      • Excluding VAT: €83,-
       0 In stock: Dropshipping: usually within 2 working days
      The eDNA Isolation Kit is used to quickly and efficiently isolate eDNA from water samples.... Show more
      6 variations Number: 20 isolates, Yes/no IPC: Without internal positive control
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      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control
      eDNA Isolation Kit - With or Without Internal Positive Control

      Product description

      The Environmental DNA Isolation Kit is used to quickly and efficiently isolate environmental DNA from water samples. The Environmental DNA Isolation Kit procedure is suitable for qualitative and quantitative PCR amplification. The whole procedure for isolation of total eDNA takes about 2 hours. The isolated DNA will be dissolved in TE and is ready to use in qPCR.

      The kit is perfectly suited for eDNA extraction and isolation from the eDNA Dual Filter membrane. See a short tutorial how to extract preserved eDNA from the capsule and how to proceed. The complete procedure is described in the instruction protocol.

      Optional:  internal positive control (IPC) quantification kit

       All Sylphium sample sets contain an internal positive control (IPC) in the preservation solution. The internal control consists of a small piece of synthetic xenobiotic DNA. The positive control DNA sequence selected is unknown in the aquatic environment and will not interfere with the detection of target organisms. The internal positive control is an efficiency check of the DNA isolation procedure and a quality and purity check of the isolated DNA. Optionally, the Internal Positive Control (IPC) quantification kit can be ordered along with the eDNA Isolation Kit. The IPC is quantified through the FAM channel.

      Kit contents DNA isolation kit

      • S1: Internal negative control
      • S2: Phase separation solution
      • S3: Precipitation buffer
      • S4: Precipitation solution
      • S5: Wash solution
      • S6: eDNA conservation buffer

      Kit contents Internal positive control qPCR detection kit (Optional)

      • Positive control
      • eTaq qPCR mastermix
      • Primer/probe mix for detection of the internal positive control (FAM dye)
      • eTaq DNA polymerase

      Equipment and consumables required

      • qPCR machine  capable for detection of the FAM dye (optional)
      • Microcentrifuge (13,000 x g)
      • Pipettors
      • -20 °Freezer
      • PCR cooling rack (optional)
      • Centrifuge for 15 ml tubes
      • Incubator or heat block at 55 °C
      • 15 ml tubes with cap
      • 2 ml tubes with lit
      • 96 wells qPCR plates + seal cover (optional)
      Tags:biology (118)ecology (131)eDNA (41)research (156)science (108)

      Specifications

      Article number
      SYL002/20/000

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      eDNA Isolation Kit - With or Without Internal Positive Control
      € 100,43
      eDNA Isolation Kit - With or Without Internal Positive Control
      Number: 20 isolates, Yes/no IPC: Without internal positive control
      0 In stock: Dropshipping: usually within 2 working days
      Add to cart

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