DNA (meta)barcoding master mix
- Inklusive 21% MwSt.: €369,05
- Ohne MwSt: €305,-
- Versandkosten nur 7,50 € nach Deutschland
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The DNA (meta)barcoding master mix for environmental DNA metabarcoding and DNA-based identification of individuals. The PCR reactions will be performed on a qPCR machine with a dye with similar to SYBR Green™, followed by a dissociation curve assay for quality control. Alternatively, a regular PCR machine can be used followed by gel electrophoresis as quality control. The DNA barcoding master mix offers excellent performance for sensitive, robust and accurate DNA amplification with a low error rate. The barcode PCR mix is highly resistant to inhibitory factors such as humic acids present in environmental samples. The barcoding master mix is based on a Pfu DNA polymerase from Pyrococcus furiosus with a modified environmental PCR mix containing a dye similar to SYBR Green™
Pfu DNA polymerase has superior thermostability and proofreading properties compared to Taq DNA polymerase. Pfu DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity by removing nucleotides that are mis incorporated. Pfu DNA polymerase results in an error rate of only 1 in 1.3 million base pairs, making it perfect for studies based on (meta) barcoding.
The DNA (meta)barcoding master mix has:
Kit contents:
The kit contains materials for 200 reactions.
Equipment and additional kits required
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